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US 8,975,384 B2Granted
Tag peptide and use thereof
AssigneeOsaka University
InventorJunichi Takagi
Life of this patent
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Abstract
The present invention provides a tag peptide comprising an amino acid sequence represented by the following formula (I): X1-Tyr-X2-Gly-Gln-X3 (I) (wherein X1, X2 and X3 are the same or different and each represent any amino acid residue) and an antibody against the tag peptide. By combined use of the tag peptide and antibody of the present invention, a system that enables proteins expressed from cloned genes to be highly purified in an inexpensive and easy manner can be established.
Claims8
An isolated tag peptide selected from one of the following (1) or (2):
- (1) an isolated tag peptide consisting of the amino acid sequence represented by (Tyr-X2-Gly-Gln:
- SEQ ID NO:96)m, wherein X2 represents Pro, Ser, Val, Ala, Thr, or Asp, amino acid residues 2-5 of SEQ ID NOs:46, 47, 58, 67, 86 or 88 and m represents an integer of 3 to 5;
- or
- (2) an isolated tag peptide consisting of the above (1) and one hydrophobic amino acid added to the C-terminus thereof.
1 more element
Dependencies are inferred from the claim text.