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US 8,969,002 B2Granted
Methods and systems for electronic sequencing
AssigneeGenapsys, Inc.
InventorsHesaam Esfandyarpour, Mark Oldham
Life of this patent
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Abstract
The present invention provides for methods and systems for Electronic DNA sequencing, single molecule DNA sequencing, and combinations of the above, providing low cost and convenient sequencing.
Claims17
A method for sequencing a nucleic acid template, comprising:
- a) contacting the nucleic acid template hybridized to a primer and coupled to a particle, which particle is proximate to a nanosensor in a nanosensor array, with a fluid containing nucleotides, wherein said nanosensor comprises a pair of electrodes;
- b) using the pair of electrodes to detect a change in conductivity within the Debye length of the particle upon incorporation of at least one nucleotide into a growing nucleic acid strand that is complementary to the nucleic acid template;
- c) washing said nanosensor array to remove excess nucleotides;
- and
- d) repeating steps a, b, and c to obtain sequence information for said nucleic acid template.
2 more elements
A method for sequencing a nucleic acid template, comprising:
- a) contacting the nucleic acid template hybridized to a primer and coupled to a particle, which particle is proximate to a nanosensor in a nanosensor array, with a fluid containing nucleotides, wherein said nanosensor comprises a pair of electrodes;
- b) using the pair of electrodes to detect a local change in charge within the Debye length of the particle upon incorporation of at least one nucleotide into a growing nucleic acid strand that is complementary to the nucleic acid template;
- c) washing said nanosensor array to remove excess nucleotides;
- and
- d) repeating steps a, b, and c to obtain sequence information for said nucleic acid template.
2 more elements
Dependencies are inferred from the claim text.