Back to results
US 8,962,292 B2Granted
Capping-prone RNA polymerase enzymes and their applications
AssigneeEukarys
InventorPhilippe Jais
Life of this patent
Estimating the term…
Abstract
The invention provides a chimeric enzyme comprising at least one catalytic domain of a RNA triphosphatase, at least one catalytic domain of a guanylyltransferase, at least one catalytic domain of a N7-guanine methyltransferase, and at least one catalytic domain of a DNA-dependant RNA polymerase. The invention also provides pharmaceutical composition comprising said chimeric enzyme and uses of said chimeric enzyme.
Claims34
A hetero-oligomeric enzyme comprising:
- a first monomer and a second monomer linked together covalently or noncovalently, the first monomer comprising
- at least one catalytic domain of a RNA triphosphatase,
- at least one catalytic domain of a guanylyltransferase, and
- at least one catalytic domain of a N7-guanine methyltransferase, and the second monomer comprising
- at least one catalytic domain of a DNA-dependent RNA polymerase, wherein the hetero-oligomeric enzyme is non-natural.
2 more elements
A hetero-oligomeric enzyme comprising:
- a dimer and a monomer linked together covalently or noncovalently, the dimer comprising:
- at least one catalytic domain of a RNA triphosphatase,
- at least one catalytic domain of a guanylyltransferase, and
- at least one catalytic domain of a N7-guanine methyltransferase, and the monomer comprising
- at least one catalytic domain of a DNA-dependent RNA polymerase, wherein the hetero-oligomeric enzyme is non-natural.
2 more elements
A monomeric enzyme comprising:
- at least one catalytic domain of a RNA triphosphatase,
- at least one catalytic domain of a guanylyltransferase,
- at least one catalytic domain of a N7-guanine methyltransferase, and
- at least one catalytic domain of a DNA-dependent RNA polymerase, wherein the monomeric enzyme is non-natural.
1 more element
Dependencies are inferred from the claim text.