Dual-pore device
AssigneeThe Regents of the University of California
InventorsWilliam Dunbar, Jungsuk Kim
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Abstract
Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide.
Claims29
A dual-pore, dual-amplifier device for simultaneously controlling the movement of a charged polymer through a first and a second pore, comprising:
- an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through the first pore, and the middle chamber is in communication with the lower chamber through the second pore, wherein the device further comprises a power supply configured to provide a first voltage between the upper chamber and the middle chamber, and provide a second voltage between the middle chamber and the lower chamber, each voltage being independently adjustable, and wherein the device provides dual-amplifier electronics configured for independent voltage control and current measurement at each pore, wherein the two voltages are different in magnitude, and wherein the first and second pores are configured so that the charged polymer is capable of simultaneously moving across both pores in either direction and in a controlled manner,
- wherein the first pore and second pore are about 10 nm to about 100 nm apart from each other.
Dependencies are inferred from the claim text.