Nanocomposite based biosensors and related methods
AssigneeIndian Institute of Technology Madras
InventorsRamaprabhu Sundara, Tessy Theres Baby
Life of this patent
Estimating the term…
Abstract
Disclosed are nanocomposite-based biosensors. The biosensors include an electrode, a nanocomposite over the surface of the electrode, the nanocomposite comprising a population of carbon nanotubes and a population of magnetic nanoparticles dispersed in the population of carbon nanotubes, wherein the magnetic nanoparticles comprise a ferromagnetic metal or compound thereof, and one or more biomolecules over the surface of the electrode, wherein the biomolecules are capable of undergoing a redox reaction with a target molecule. Also disclosed are nanocomposites, modified electrodes, kits, and methods for using the biosensors.
Claims19
A biosensor comprising:
- an electrode having a surface;
- a nanocomposite on the surface of the electrode, the nanocomposite comprising magnetic nanoparticles dispersed on an outer surface of each member of a population of carbon nanotubes,
- wherein the magnetic nanoparticles comprise core-shell magnetic nanoparticles comprising a core comprising a ferromagnetic metal or compound thereof, and a shell comprising silicon dioxide;
- and
- one or more biomolecules over the surface of the electrode, wherein the biomolecules are configured to undergo a redox reaction with a target molecule;
- wherein the outer surface of the carbon nanotubes comprise one or more functional groups covalently bonded to the outer surface.
A nanocomposite comprising:
- a population of carbon nanotubes;
- and
- a population of magnetic nanoparticles dispersed on the outer surface of each member of the population of carbon nanotubes;
- wherein:
- the magnetic nanoparticles comprise core-shell magnetic nanoparticles;
- and
- the core comprises a ferromagnetic metal or a compound thereof;
- wherein the outer surface of the carbon nanotubes comprise one or more functional groups covalently bonded to the outer surface, wherein the one or more functional groups are selected from the group consisting of COOH, OH, NH, NH2, F, COX, or SH, wherein X is F, Cl, Br, or I.
A method for determining a concentration of a target molecule in a sample, the method comprising:
- exposing the sample to a biosensor, the biosensor comprising an electrode;
- a nanocomposite on the surface of the electrode, the nanocomposite comprising magnetic nanoparticles dispersed on an outer surface of each member of a population of carbon nanotubes,
- wherein the magnetic nanoparticles comprise core-shell magnetic nanoparticles comprising a core comprising a ferromagnetic metal or compound thereof, and a shell comprising silicon dioxide;
- and
- one or more biomolecules over the surface of the electrode, wherein the biomolecules are configured to undergo a redox reaction with a target molecule, wherein the outer surface of the carbon nanotubes comprise one or more functional groups covalently bonded to the outer surface, and
- detecting a signal from the biosensor, wherein the signal is correlated to the concentration of the target molecule in the sample.
Dependencies are inferred from the claim text.