Process for decreasing verbascose in a plant by expression of a chloroplast-targeted fimD protein

A process for producing a plant cell, plant or part thereof with a decrease in the amount of verbascose relative to a corresponding control plant cell, plant or part thereof, which comprises

• a) introducing into a chloroplast of a plant cell, plant, or a part thereof a nucleic acid comprising
• i) the nucleic acid sequence of SEQ ID NO:
• 71513;
• ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO:
• 71514;
• iii) a nucleic acid sequence encoding an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 71514;
• or
• iv) a nucleic acid which hybridizes with the complement of the nucleic acid of (i) under high stringent hybridization conditions of about 0.2×SSC at 50° C. to 65° C.;
• b) introducing into a plant cell, plant, or a part thereof a nucleic acid comprising a nucleic acid operably linked to a polynucleotide encoding a transit peptide, wherein the nucleic acid comprises
• i) the nucleic acid sequence of SEQ ID NO:
• 71513;
• ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO:
• 71514;
• iii) a nucleic acid sequence encoding an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 71514;
• or
• iv) a nucleic acid which hybridizes with the complement of the nucleic acid of (i) under high stringent hybridization conditions of about 0.2×SSC at 50° C. to 65° C.;
• or
• c) introducing into a plant cell, plant, or a part thereof a nucleic acid operably linked to a polynucleotide encoding a chloroplast localization sequence, wherein the nucleic acid comprises
• i) the nucleic acid sequence of SEQ ID NO:
• 71513;
• ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO:
• 71514;
• iii) a nucleic acid sequence encoding an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 71514;
• or
• iv) a nucleic acid which hybridizes with the complement of the nucleic acid of (i) under high stringent hybridization conditions of about 0.2×SSC at 50° C. to 65° C.;
• wherein expression of the nucleic acid results in a decrease in the amount of verbascose relative to a corresponding control plant cell, plant or part thereof.

An expression cassette comprising a promoter that functions in plants operably linked to a nucleic acid encoding a fusion protein, wherein the fusion protein comprises a chloroplast transit peptide and a polypeptide comprising

• i) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
• 71514;
• ii) the amino acid sequence of SEQ ID NO:
• 71514;
• or
• iii) an amino acid sequence encoded by a nucleic acid molecule which hybridizes under high stringent hybridization conditions of about 0.2×SSC at 50° C. to 65° C. with the complement of a nucleic acid molecule comprising the sequence of SEQ ID NO:
• 71513;
• wherein the expression cassette when expressed in a plant cell, plant, or part thereof results in a decrease in the amount of verbascose relative to a corresponding control plant cell, plant, or part thereof.

A process for producing a polypeptide in a plant cell, plant, or part thereof, comprising:

• (a) obtaining a plant cell, plant, or part thereof transformed stably or transiently with a nucleic acid molecule;
• and
• (b) producing a polypeptide encoded by said nucleic acid molecule in said plant cell, plant, or part thereof,
• wherein said polypeptide is expressed in, or targeted to, a chloroplast of said plant cell, plant, or part thereof,
• wherein said nucleic acid molecule is:
• (i) a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
• 71514;
• (ii) a nucleic acid molecule comprising the nucleic acid molecule of SEQ ID NO:
• 71513;
• (iii) a nucleic acid molecule encoding a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
• 71514;
• or
• (iv) a nucleic acid molecule which hybridizes with the complement of the nucleic acid molecule of (ii) under high stringent hybridization conditions of about 0.2×SSC at 50° C. to 65° C.,
• and wherein expression of said nucleic acid molecule in said plant cell, plant, or part thereof confers a decrease in the amount of verbascose relative to a corresponding control plant cell, plant, or part thereof.