Elite event A5547-127 and methods and kits for identifying such event in biological samples

A primer pair comprising a first primer having a sequence which, under optimized PCR conditions specifically recognizes a sequence within the 5′ flanking region of A5547-127, and a second primer which, under optimized PCR conditions specifically recognizes a sequence within the foreign DNA contiguous with said 5′ flanking region, or comprising a first primer which, under optimized PCR conditions specifically recognizes a sequence within the 3′ flanking region of A5547-127, and a second primer which, under optimized PCR conditions specifically recognizes a sequence within the foreign DNA contiguous with said 3′ flanking region, wherein said 5′ flanking region comprises the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311 and said 3′ flanking region having the nucleotide sequence of the complement of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, said foreign DNA contiguous with said 5′ flanking region comprises the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 312 to nucleotide 810, and said foreign DNA contiguous with said 3′ flanking region comprises the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 509.

A primer pair comprising a first primer comprising at its extreme 3′ end the sequence of SEQ ID No. 13, and a second primer comprising at its extreme 3′ end the sequence of SEQ ID No. 15.

A kit comprising a specific probe, capable of hybridizing specifically to a specific region of A5547-127, wherein said specific region is a DNA comprising part of the 5′ flanking sequence of A5547-127 and the sequence of the foreign DNA contiguous therewith, or is a DNA comprising part of the 3′ flanking sequence of A5547-127 and the sequence of the foreign DNA contiguous therewith,

• wherein said 5′ flanking region comprises the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311, said 3′ flanking region comprising the nucleotide sequence of the complement of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, wherein said foreign DNA contiguous with said 5′ flanking region comprises the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 312 to nucleotide 810, and wherein said foreign DNA contiguous with said 3′ flanking region comprises the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 509, or the complement of said sequences, and
• wherein the sequence of said specific probe has at least 80% sequence identity with SEQ ID No. 1 from nucleotide 260 to 360 or SEQ ID No. 2 from nucleotide 460 to 560, or the complement of said sequences.

A specific probe comprising a sequence which has at least 80% sequence identity with a sequence comprising part of the 5′ or 3′ flanking sequence of A5547-127 and the sequence of the foreign DNA contiguous therewith, or the complement thereof,

• wherein said 5′ flanking region comprises the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311, said 3′ flanking region comprising the nucleotide sequence of the complement of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, wherein said foreign DNA contiguous with said 5′ flanking region comprises the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 312 to nucleotide 810, and wherein said foreign DNA contiguous with said 3′ flanking region comprises the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 509, or the complement of said sequences,
• wherein the sequence of said specific probe has at least 80% sequence identity with SEQ ID No. 1 from nucleotide 260 to 360 or SEQ ID No. 2 from nucleotide 460 to 560, or the complement of said sequences.

A specific probe comprising the nucleotide sequence of SEQ ID No. 1 from nucleotide 260 to 360 or of SEQ ID No. 2 from nucleotide 460 to 560, or the complement of said sequences.

A DNA comprising the 5′ flanking region of the A5547-127 event and the foreign DNA contiguous therewith, wherein said DNA comprises a sequence of between 50 and 500 bp with at least 80% sequence identity to a DNA comprising the 5′ flanking region of the A5547-127 event and the foreign DNA contiguous therewith, said 5′ flanking region comprising the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 311, and said foreign DNA contiguous therewith comprising the nucleotide sequence of SEQ ID No. 1 from nucleotide 312 to nucleotide 810;

• wherein said DNA comprises the sequence of SEQ ID No. 1 from nucleotide 260 to nucleotide 360.

A DNA comprising the 3′ flanking region of the A5547-127 event and the foreign DNA contiguous therewith,

• wherein said DNA comprises a sequence with at least 80% sequence identity to a DNA comprising the 3′ flanking region of the A5547-127 event and the foreign DNA contiguous therewith, said 3′ flanking region comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 510 to nucleotide 1880, and said foreign DNA contiguous therewith comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 509;
• wherein said DNA contains the nucleotide sequence of SEQ ID No. 1 from nucleotide position 460 to nucleotide position 560.

An isolated DNA comprising a nucleotide sequence with at least 95% sequence identity to the nucleotide sequence of SEQ ID No 1 from nucleotide 260 to nucleotide 360.

An isolated DNA comprising a nucleotide sequence with at least 95% sequence identity to the nucleotide sequence of SEQ ID No 2 from nucleotide 460 to nucleotide 560.

An isolated DNA comprising the nucleotide sequence of SEQ ID No 1 from nucleotide 260 to nucleotide 360.

An isolated DNA comprising the nucleotide sequence of SEQ ID No 2 from nucleotide 460 to nucleotide 560.