US 8,946,511 B2Granted

Plant microRNAs and methods of use thereof

AssigneeMonsanto Technology LLC

InventorsEdwards Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalik, Elysia K. Krieger, James K. Roberts, Yuanji Zhang

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Abstract

This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

Claims6

Dependent claims

A recombinant DNA construct comprising a heterologous promoter functional in a plant cell and operably linked to DNA encoding an RNA transcript consisting essentially of at least one miRNA decoy sequence that is recognized and bound by an endogenous mature plant miRNA but not cleaved, wherein

said miRNA decoy sequence is an RNA sequence of between about 19 to about 36 contiguous RNA nucleotides, wherein said miRNA decoy sequence is recognized and bound by said endogenous mature plant miRNA, resulting in base-pairing between said miRNA decoy sequence and said endogenous mature plant miRNA, thereby forming a cleavage-resistant RNA duplex comprising at least one mismatch between said miRNA decoy sequence and said endogenous mature plant miRNA at positions 9, 10, or 11 of said endogenous mature plant miRNA, or at least one insertion at a position in said miRNA decoy sequence corresponding to positions 10-11 of said endogenous mature plant miRNA.

Dependencies are inferred from the claim text.

Facts

Application: 11/974,469Filed 12 Oct 2007 · us provisional application
Priority: 12 Oct 2006
Published: US 2009/0070898 A112 Mar 2009
Granted: 3 Feb 2015
Term ends: Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12N 15/113
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: non-coding nucleic acids modulating the…
C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells
C12N 15/8218
Methods for controlling, regulating or enhancing expression of transgenes in plant cells: antisense, co-suppression, viral induced gene silencing…
C12N 15/8238
Externally regulated expression systems: chemically inducible, e.g. tetracycline

Family

Application60/851,187Us provisional application · filed 12 Oct 2006
Application60/908,826Us provisional application · filed 29 Mar 2007
Application60/969,195Us provisional application · filed 31 Aug 2007
US 2009/0070898 A1Pre-grant publication · published 12 Mar 2009
US 8,946,511 B2This patent

From the US filing history. Foreign family members aren’t listed.