Plants having tomato torrado virus resistance
AssigneeMonsanto Invest B.V.
InventorsPaulus Cornelis Maris, Anita Afke de Haan, Johannes Hendrikus Maria Barten, Johannes Franciscus Johanna Maria van den Heuvel
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Abstract
The present invention relates to a tomato plant having within its genome at least one allele of a gene that confers resistance to Tomato torrado virus (ToTV), said virus having been deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH on 24 Nov. 2004 under depositors reference number ToTV-E01 (DSM 16999).
Claims17
A hybrid tomato plant of the species Solanum lycopersicum comprising commercially desirable characteristics, obtained by crossing a donor plant having within its genome a haplotype that confers resistance to tomato torrado virus (ToTV), as found in tomato breeding line T06PF1, a strain of said virus having been deposited with the Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH on 24 Nov. 2004 under depositor's reference number ToTV-E01 (DSM 16999), and a deposit of seed of said tomato line having been deposited with the American type Culture Collection (ATCC) on 6 Mar. 2014 under depositor's reference number 11 EFT 41822 (PTA-121067), wherein said haplotype is located in a genomic region of chromosome 4 between markers CT264 and TG163, linked to one or more markers selected from the group consisting of P14/M49-F-282, P11/M54-F-233/235, P11/M35-F-216, P21/M61-F-583, P25/M51-F-131, P015/M49-F-330, P13/M38-F-311/313 and C2_At5g25900, with a recipient plant comprising commercially desirable characteristics, wherein said obtained plant is produced by a method comprising screening for the presence of at least one molecular marker linked to said ToTV resistance haplotype, and wherein said haplotype is present in homozygous form.
A tomato plant of the species Solanum lycopersicum obtained by crossing a donor plant having within its genome a haplotype as found in tomato breeding line T06PF1, a deposit of seed of said tomato line having been deposited with the American type Culture Collection (ATCC) on 6 Mar. 2014 under depositor's reference number 11 EFT 41822 (PTA-121067), in homozygous form that confers resistance to tomato torrado virus (ToTV) with a recipient plant comprising commercially desirable characteristics, wherein said haplotype is located in a genomic region of chromosome 4 between markers CT264 and TG163 linked to one or more markers selected from the group consisting of P14/M49-F-282, P11/M54-F-233/235, P11/M35-F-216, P21/M61-F-583, P25/M51-F-131, P15/M49-F-330, P13/M38-F-311/313 and C2_At5g25900, wherein said obtained plant is obtained by
- a) providing an inoculum of said ToTV;
- b) exposing a tomato plant to said inoculum;
- c) allowing for a sufficient incubation time;
- and
- d) selecting said tomato plant having within its genome the haplotype that confers resistance to ToTV if no infection establishes in said plant upon lapse of said incubation time.
Dependencies are inferred from the claim text.