Multi-targeting short interfering RNAs
AssigneeCity of Hope
InventorsJohn J. Rossi, Ola Snove, Jr., Ali Ehsani, Pal Saetrom, Britta Hoehn, Jane Zhang, Lars Aagaard
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Abstract
The present invention relates to novel short interfering RNA (siRNA) molecules that are multi-targeted. More specifically, the present invention relates to siRNA molecules that target two or more sequences. In one embodiment, multi-targeting siRNA molecules are designed to incorporate features of siRNA molecules and features of micro-RNA (miRNA) molecules. In another embodiment, multi-targeting siRNA molecules are designed so that each strand is directed to separate targets.
Claims10
A multi-targeting RNA molecule that targets two or more different desired target sequences, wherein the RNA molecule comprises a first strand having a 5′ terminus and a 3′ terminus and a second strand having a 5′ terminus and a 3′ terminus, wherein the first strand is perfectly complementary to a desired mRNA target sequence, wherein the second strand contains two or more seed matches optimally spaced in a desired 3′ UTR containing target gene, wherein the first strand and second strand are not completely complementary and wherein the first strand and the second strand hybridize to form a double stranded RNA molecule, wherein the double stranded RNA molecule is a single duplex.
Dependencies are inferred from the claim text.