Pathogen-inducible synthetic promoter
AssigneeKws Saat AG
InventorKlaus Schmidt
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Abstract
The invention relates to a pathogen-inducible synthetic promoter which is suitable for regulating the transcription of a nucleic acid, and includes a minimal promoter, characterized in that the minimal promoter includes a sequence motif a) dbrmwa or b) twcccmt which is disposed downstream from a TATA region and in front of a transcription starting point which is located on the minimal promoter and at which transcription of the nucleic acid to be regulated starts.
Claims21
A pathogen inducible synthetic promoter for regulating the transcription of a nucleic acid, which includes at least one cis regulatory element and a minimal promoter,
- wherein the minimal promoter is heterologous to the at least one cis regulatory element,
- wherein the minimal promoter consists essentially of a TATA-box, a transcription starting point, and a sequence motif dbrmwa, and
- wherein the sequence motif dbrmwa is disposed downstream from the TATA-box and in front of the transcription starting point at which transcription of the nucleic acid to be regulated starts.
A vector or an expression cassette comprising a minimal promoter for producing a pathogen inducible synthetic promoter, wherein the minimal promoter consists essentially of a TATA-box, a transcription starting point, and a sequence motif dbrmwa, wherein the sequence motif dbrmwa is disposed downstream from the TATA-box and in front of the transcription starting point at which transcription of a nucleic acid to be regulated starts, and wherein the TATA-box is the binding location for the TATA-box binding protein (TBP) associated with transcription of said nucleic acid, and wherein the minimal promoter is operably linked to at least one cis regulatory element.
A pathogen inducible synthetic promoter, which is suitable for regulating the transcription of a nucleic acid, and includes at least one cis regulatory element and a minimal promoter,
- wherein the minimal promoter is heterologous to the cis regulatory element,
- wherein the minimal promoter consists essentially of a TATA-box, a transcription starting point, and a sequence motif dbrmwa,
- wherein the sequence motif dbrmwa is disposed downstream from the TATA-box and in front of the transcription starting point at which transcription of the nucleic acid to be regulated starts, and
- which includes, in addition to the minimal promoter, at least one cis-regulating element with the nucleotide sequence according to one of SEQ ID NOS:
- 10-15.
A pathogen inducible synthetic promoter for regulating the transcription of a nucleic acid, which includes at least one cis regulatory element and a minimal promoter, wherein the minimal promoter is heterologous to the at least one cis regulatory element and wherein the minimal promoter comprises a sequence motif dbrmwa which is disposed downstream from a TATA-box and in front of a transcription starting point which are located on the minimal promoter and at which transcription of the nucleic acid to be regulated starts, and wherein the TATA-box is the binding location for the TATA-box binding protein (TBP) associated with transcription of said nucleic acid.
Dependencies are inferred from the claim text.