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AssigneeAbbVie Inc.
InventorsMatthew Omon Herigstad, Linda E. Rich, Stephen Ming-teh Lu, Natarajan Ramasubramanyan
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The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.
A method for producing a preparation comprising a protein of interest and having a reduced level of at least one impurity, said method comprising:
A method for producing a preparation comprising adalimumab and having a reduced level of at least one impurity, said method comprising:
Dependencies are inferred from the claim text.