Medium comprising transforming growth factor β1 and basic fibroblast growth factor
AssigneeTechnion Research & Development Foundation Limited
InventorsMichal Amit, Joseph Itskovitz-Eldor
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
Claims24
A culture medium which comprises transforming growth factor β1 (TGFβ1), and basic fibroblast growth factor (bFGF), wherein the culture medium is free of animal- and/or feeder cells contaminants, and said culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
A culture medium comprising transforming growth factor β1 (TGFβ1), and basic fibroblast growth factor (bFGF), wherein the culture medium comprises serum replacement, wherein said culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
A culture medium comprising transforming growth factor β1 (TGFβ1), and basic fibroblast growth factor (bFGF), animal-free albumin and being devoid of serum, wherein said culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
A culture medium comprising transforming growth factor β1 (TGFβ1), and basic fibroblast growth factor (bFGF), wherein the culture medium comprises albumin or albumin substitutes, amino acids, vitamins, transferrins or transferrin substitutes, antioxidants, insulin or insulin substitutes, collagen precursors and trace elements, wherein said culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
A culture medium comprising transforming growth factor β1 (TGFβ1), and basic fibroblast growth factor (bFGF) and being devoid of serum, wherein the culture medium comprises serum replacement, wherein said culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
A culture medium comprising transforming growth factor β1 (TGFβ1), and basic fibroblast growth factor (bFGF) and being devoid of serum, wherein the culture medium comprises albumin or albumin substitutes, amino acids, vitamins, transferrins or transferrin substitutes, antioxidants, insulin or insulin substitutes, collagen precursors and trace elements, wherein said culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
Dependencies are inferred from the claim text.