Process and culturing unit for culturing biological cells
AssigneeFraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
InventorsGünter R. Fuhr, Hagen Von Briesen, Erwin Gorjup, Charli Kruse
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Abstract
A targeted cultivation phase adjustment is provided in a process for the cultivation of cells in which biological cells are cultivated on at least one boundary surface between different, non-solid cultivation phases so that pre-determined cultivation conditions are given. A cultivation apparatus (100) for biological cells is also provided that includes a culture container (10) with different, non-solid cultivation phases (20) between which at least one boundary surface is formed.
Claims18
A process for cell cultivation of biological cells comprising the steps of:
- a) introducing an outer cultivation phase and an inner cultivation phase into a culture container, wherein the outer cultivation phase surrounds the inner cultivation phase and a curved boundary surface is formed by the outer cultivation phase and inner cultivation phase, and wherein:
- i) the outer cultivation phase is a silicone oil or gel, and the inner cultivation phase is a nutrient medium;
- or
- ii) the outer cultivation phase is a nutrient medium, and the inner cultivation phase is a silicone oil;
- and
- b) culturing the biological cells at the curved boundary surface wherein the biological cells are introduced during or after the introducing of the inner cultivation phase.
A process for cell cultivation of biological cells comprising the steps of:
- a) introducing into a culture container an outer cultivation phase and an inner cultivation phase surrounded by the outer cultivation phase, providing a membrane on a curved boundary surface between the outer cultivation phase and the inner cultivation phase, and wherein:
- i) the outer cultivation phase is a silicone oil or gel and the inner cultivation phase is a nutrient medium;
- or
- ii) the outer cultivation phase is a nutrient medium, and the inner cultivation phase is a silicone oil or gel;
- and
- b) culturing biological cells at the membrane, wherein the biological cells are introduced during or after the introducing of the inner cultivation phase.
Dependencies are inferred from the claim text.