Methods and composition for treating neural degeneration
AssigneeSan Bio, Inc.
InventorsKeita Mori, Martha C. Bohn, Ciara Tate, Irina Aizman, Aleksandra Glavaski, Tamas Virag
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Abstract
Disclosed herein are methods and compositions for the use of marrow adherent stem cells and their descendents; e.g., bone marrow-derived neural regenerating cells; in the treatment of various neurodegenerative disorders. In certain embodiments, bone marrow-derived neural regenerating cells transplanted to sites of neural degeneration stimulate growth and/or survival of host neurons.
Claims21
A method for promoting survival of neural cells in a host by provision of one or more trophic factors, wherein the method comprises transplanting bone marrow-derived neural regenerating cells at or near a site of neural degeneration in the host, wherein the neural regenerating cells are obtained by a process comprising the steps of:
- (a) providing a culture of marrow adherent stromal cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD), wherein the NICD consists of amino acids 1703-2504 of the Notch-1 protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and
- (d) further culturing the selected cells of step (c) in the absence of selection;
- and further wherein the neural regenerating cells provide trophic factors that promote survival of the neural cells in the host.
A method for stimulating growth of neural cells in a host by provision of one or more trophic factors, wherein the method comprises transplanting bone marrow-derived neural regenerating cells at or near a site of neural degeneration in the host, wherein the neural regenerating cells are obtained by a process comprising the steps of:
- (a) providing a culture of marrow adherent stromal cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD), wherein the NICD consists of amino acids 1703-2504 of the Notch-1 protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and
- (d) further culturing the selected cells of step (c) in the absence of selection;
- and further wherein the neural regenerating cells provide trophic factors that stimulate growth of the neural cells in the host.
A method for preventing death of neural cells in a host by provision of one or more trophic factors, wherein the method comprises transplanting bone marrow-derived neural regenerating cells at or near, a site of neural degeneration in the host, wherein the neural regenerating cells are obtained by a process comprising the steps of:
- (a) providing a culture of marrow adherent stromal cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD), wherein the NICD consists of amino acids 1703-2504 of the Notch-1 protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and
- (d) further culturing the selected cells of step (c) in the absence of selection;
- and further wherein the neural regenerating cells provide trophic factors that prevent death of the neural cells in the host.
Dependencies are inferred from the claim text.