Enhanced surface area conico-cylindrical flask (ES-CCF) for biofilm cultivation

An enhanced surface area conico-cylindrical flask (ES-CCF) for biofilm cultivation of a biofilm-forming microorganism comprising:

• a funnel portion;
• a lower cylindrical portion being closed at a lower end and open at a upper end with the upper end comprising a middle threading portion for joining the funnel portion and lower cylindrical portion;
• a neck being detachably mounted at a top of the funnel portion;
• a screw cap having two openings each for accepting a port with a first port being an air inlet port and a second port being an exhaust port, the screw cap being detachably fitted onto the neck;
• wherein the lower cylindrical portion accepts an inner arrangement having a plurality of equally spaced rectangular strips directly mounted onto a circular disk;
• wherein the inner arrangement is removably placed inside the lower cylindrical portion with the circular disk positioned at the lower end of the lower cylindrical portion;
• wherein each rectangular strip of the inner arrangement is orientated outward from a central axis and orientated perpendicular to a tangent of the circumference of the lower cylindrical portion;
• wherein a long side of each rectangular strip is parallel to the axis between the lower end and the upper end of the lower cylindrical portion;
• and
• wherein at least one of the lower cylindrical portion, the rectangular strips of the inner arrangement, and the circular disk of the inner arrangement is made of polymethylmethacrylate (PMMA).