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AssigneeCj Cheiljedang Corporation
InventorsJae-yeong Ju, Hyang Choi, Eun-sung Koh, Ji-sun Lee, Jin-ho Lee, So-young Kim, Chang-hyun Jin, Young-hoon Park
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The present invention relates to a microorganism having L-tryptophan productivity and a method for producing L-tryptophan using the same. More precisely, the present invention relates to the recombinant E. coli strain CJ600 (KCCM 10812P) having tryptophan productivity produced from the mutant form (KFCC 10066) of E. coli having L-phenylalanine productivity, wherein tryptophan auxotrophy is released, L-phenylalanine biosynthesis is blocked but tryptophan productivity is enhanced by reinforcing the gene involved in tryptophan biosynthesis, and a method of producing L-tryptophan using the same.
A recombinant E. coli strain having L-tryptophan productivity derived from a mutant E. coli (KFCC 10066) having L-phenylalanine productivity, wherein tryptophan biosynthetic genes trpA-E are overexpressed at an expression level greater than or equal to a minimum expression level necessary for growth in a tryptophan free medium, and pheA, trpR, mtr and tnaAB genes are inactivated and aroG and trpE genes are mutated on the chromosome of said mutant E. coli such that the 449th nucleotide of the aroG gene is substituted with thymine and the 61st nucleotide of the trpE gene is substituted with thymine.
A recombinant E. coli strain having L-tryptophan productivity derived from a mutant E. coli (KFCC 10066) having L-phenylalanine productivity, wherein tryptophan biosynthetic genes trpA-E are overexpressed at an expression level greater than or equal to a minimum expression level necessary for growth in a tryptophan free medium, and pheA, trpR, mtr and tnaAB genes are inactivated and aroG and trpE genes are mutated on the chromosome of said mutant E. coli such that the 150th amino acid of the protein product of the aroG gene is substituted with leucine and the 21st amino acid of the protein product of the trpE gene is substituted with serine.
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