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US 8,945,904 B2Granted
Influenza virus reassortment
AssigneeNovartis AG
InventorsHeidi Trusheim, Peter Mason, Michael Franti, Bjoern Keiner, Melissa Sackal, Juerg Hunziker, Francois Natt, David Morrissey
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Abstract
Methods for producing reassortant viruses are provided wherein the transcription and/or translation of the hemagglutinin and/or neuraminidase genes are suppressed.
Claims28
A method of preparing a reassortant influenza virus comprising the steps of
- (i) contacting a culture host infected with a vaccine strain and a backbone strain with a nucleic acid inhibitory agent wherein said inhibitory agent preferentially reduces the transcription and/or translation of the hemagglutinin and/or neuraminidase genes of the backbone strain;
- and,
- (ii) culturing the culture host in order to produce the reassortant virus.
A method of preparing a reassortant influenza virus comprising the steps of
- (i) introducing into a culture host that has been infected with a first influenza virus strain having at least one target segment one or more expression construct(s) encoding the target segment(s) from a second influenza virus strain;
- (ii) contacting the culture host with a nucleic acid inhibitory agent which preferentially reduces the transcription and/or translation of the first influenza strain's target segment(s);
- (iii) culturing the culture host in order to produce reassortant virus;
- and optionally
- (iv) purifying the virus obtained in step (iii).
2 more elements
A cell for preparing a reassortant influenza virus comprising expression construct(s) encoding:
- (i) all eight viral segments of a first influenza A or B virus genome;
- (ii) at least one target segment of a second influenza A or B virus genome, wherein the second influenza strain's target segment(s) differs in sequence from the target segment of the first influenza strain;
- and
- (iii) a nucleic acid inhibitory agent wherein said inhibitory agent preferentially reduces transcription and/or translation of the target segment(s) of the first influenza strain.
1 more element
Dependencies are inferred from the claim text.