Cellobiohydrolase variants
AssigneeCodexis, Inc.
InventorsDipnath Baidyaroy, Louis Clark, David Elgart, Rama Voladri, Xiyun Zhang
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Abstract
The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.
Claims15
A recombinant cellobiohydrolase type 2a (CBH2a) variant comprising at least about 90% sequence identity to SEQ ID NO:1 and comprising an amino acid substitution, relative to SEQ ID NO:1, at one or more positions selected from 15, 204, and 340, wherein the position is numbered with reference to SEQ ID NO:1.
A recombinant cellobiohydrolase type 2 (CBH2) variant comprising at least 90% sequence identity to SEQ ID NO:1 and comprising an amino acid substitution selected from:
- a glutamine, arginine, or tryptophan residue at position 204 (X204Q/R/W);
- wherein the position is numbered with reference to SEQ ID NO:1, and wherein the cellobiohydrolase variant has increased thermostability in comparison to the wild-type cellobiohydrolase from which the variant is derived.
Dependencies are inferred from the claim text.