Variants of fungal serine protease

An isolated polypeptide variant of the Fusarium equiseti Fe_RF6318 serine protease, the polypeptide variant comprising the amino acid sequence set forth in SEQ ID NO:2 with a substitution at position 208 in SEQ ID NO:2 of an amino acid other than valine, and optionally further comprising one or more mutations selected from the group consisting of:

• (a) a substitution of one or more amino acid residues at positions 3, 6, 7, 8, 14, 17, 18, 22, 24, 25, 28, 29, 33, 34, 36, 37, 46, 47, 52, 56, 61, 63, 65, 69, 76, 77, 83, 88, 91, 100, 103, 106, 111, 113, 114, 121, 123, 138, 144, 151, 153, 155, 157, 158, 164, 167, 169, 173, 174, 175, 176, 185, 196, 205, 206, 210, 214, 216, 230, 234, 236, 239, 247, 248, 249, 252, 256, 260, 268, 281, 282, 283, 284, 286, 287, or 288 of SEQ ID NO:2 with an amino acid other than the amino acid occurring at those positions in SEQ ID NO:2;
• (b) a deletion of alanine at position 65 of SEQ ID NO:2;
• (c) a deletion of histidine at position 66 of SEQ ID NO:2;
• (d) a deletion of asparagine at position 167 of SEQ ID NO:2;
• and
• (e) an amino acid insertion after position 103 of SEQ ID NO:2,
• wherein the polypeptide variant:
• (i) has hydrolytic activity on a substrate selected from the group consisting of casein, hemoglobin, keratin, and BSA, and
• (ii) has improved thermal or detergent stability compared to a polypeptide having the amino acid sequence set forth in SEQ ID NO:2.