Recombinant host cell with deficiency in non-ribosomal peptide synthase production

A method for production of a compound of interest by microbial fermentation comprising:

• a. providing a microbial host cell,
• b. culturing said microbial host cell in a culture medium under conditions conducive to expression of the compound of interest, and
• c. isolating the compound of interest from the culture medium,
• wherein said microbial host cell has been modified in the genome thereof resulting in a deficiency in production of at least one non-ribosomal peptide synthase, wherein said microbial host cell is a filamentous fungus, and wherein said at least one non-ribosomal peptide synthase is at least 95% identical to a polypeptide selected from the group consisting of the polypeptide according to SEQ ID NO:
• 38 (npsE), and the polypeptide according to SEQ ID NO:
• 34 (Pc16g04690), and/or wherein said microbial host cell has been modified in its genome such that it results in a reduction of the amount of at least one mRNA having at least 90% identity with a mRNA selected from the group consisting of the mRNA according to SEQ ID NO:
• 37, and the mRNA according to SEQ ID NO:
• 33.