Alternating electric current directs, enhances, and accelerates mesenchymal stem cell differentiation into either osteoblasts or chondrocytes but not adipocytes
OwnerCourtney M. Creecy, Rena Biziosinventors
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Abstract
A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current.
Claims33
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells within a three dimensional matrix or scaffold;
- exposing mesenchymal stem cells to alternating electric current, wherein the alternating electric current is a current in the range from 10 μA to 40 μA.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells;
- exposing mesenchymal stem cells to alternating electric current wherein the alternating electric current is a frequency of 10 Hz.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells on a two dimensional substrate;
- exposing mesenchymal stem cells to alternating electric current, wherein the alternating electric current is of either a continuous or intermittent pattern and a duration of application of 6 hours per day for up to 28 consecutive days.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells within a three-dimensional matrix or scaffold;
- exposing mesenchymal stem cells to alternating electric current;
- and wherein the alternating electric current is a current in the range from 10 μA to 40 μA.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells within a three-dimensional matrix or scaffold;
- exposing mesenchymal stem cells to alternating electric current;
- and wherein the alternating electric current is a frequency of 10 Hz.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells within a three-dimensional matrix or scaffold;
- exposing mesenchymal stem cells to alternating electric current;
- and wherein the alternating electric current is for either a continuous or intermittent pattern and a duration of application of 6 hours per day for up to 28 consecutive days.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells in two dimensions on a substrate;
- exposing mesenchymal stem cells to alternating electric current;
- and wherein the alternating electric current is a current in the range from 10 μA to 40 μA.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells in two dimensions on a substrate;
- exposing mesenchymal stem cells to alternating electric current;
- and wherein the alternating electric current is a frequency of 10 Hz.
A method for directing, enhancing, and accelerating mesenchymal stem cell differentiation, comprising the steps of:
- culturing mesenchymal stem cells on a two-dimensional substrate;
- exposing mesenchymal stem cells to alternating electric current;
- and wherein the alternating electric current is of either a continuous or intermittent pattern and a duration of application of 6 hours per day for up to 28 consecutive days.
Dependencies are inferred from the claim text.