Method for producing monoterpene and monoterpinoid compounds and use thereof
AssigneeBoard of Regents of the Nevada System of Higher Education, on behalf of the University of Nevada, Reno
InventorsClaus Tittiger, Rubi Figueroa-Teran, Gary J. Blomquist
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Abstract
In various embodiments, the present disclosure provides a method and enzyme for forming various compounds, such as monoterpenes and monoterpenoid compounds. In a specific example, the present disclosure provides a method for producing one or more of (−)-ipsdienol, (−)-ipsenol, ipsenone, and ipsdienone. The present disclosure also provides methods of using compounds formed from the disclosed method and enzyme.
Claims12
A method of catalyzing the oxidation of ipsenol or ipsdienol comprising contacting a sample comprising ipsenol or ipsdienol with an isolated, synthetic or transgenically expressed polypeptide having oxidation activity encoded by a polynucleotide sequence at least 95% homologous to the sequence of SEQ ID NO:1.
A method of catalyzing the reduction of ipsenone or ipsdienone comprising contacting a sample comprising ipsenone or ipsdienone with an isolated synthetic or transgenically expressed polypeptide having reduction activity encoded by a polynucleotide sequence at least 95% homologous to the sequence of SEQ ID NO:1.
A process for resolving an enantiomeric mixture of ipsenol or ipsdienone comprising:
- providing an enzymatically active isolated, synthetic or transgenically expressed polypeptide having oxidation reduction or oxidoreductase activity encoded by a polynucleotide sequence at least 95% homologous to the sequence of SEQ ID NO:1;
- providing a mixture comprising at least one of ipsenol or ipsdienol;
- contacting the mixture with the polypeptide under conditions wherein the polypeptide can oxidize the alcohol;
- contacting the mixture with the polypeptide under conditions wherein the polypeptide can reduce the previously oxidized alcohol;
- and
- recovering a mixture of alcohols enriched in one enantiomer or a mixture of alcohols enriched in the opposite enantiomer.
Dependencies are inferred from the claim text.