Whole cell biocatalyst
AssigneeZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KG
InventorsJoachim Jose, Ruth Maas, Christian Detzel
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Abstract
The present invention relates to a method for producing a product of a reaction catalysed by a nitrilase, which method comprises the steps (i) providing a microorganism comprising said nitrilase located on its surface, and/or a membrane preparation of said microorganism, and (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity. The present invention further relates to a method for producing enantiomerically pure (R)-mandelic acid using the nitrilase-displaying whole cell biocatalyst or membrane preparation thereof for the conversion of racemic mandelonitrile.
Claims13
A method comprising the steps of:
- (i) providing a microorganism comprising a recombinant nitrilase located on its surface, and/or on a membrane preparation of said microorganism,
- (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity, wherein the nitrilase is fused to a transporter domain of an autotransporter.
Dependencies are inferred from the claim text.