Back to results
US 8,945,878 B2Granted
Methods for identifying and isolating cells expressing a polypeptide
AssigneeOncoMed Pharmaceuticals, Inc.
InventorsAustin L. Gurney, Alexandra L. L. Lazetic, Christopher J. Bond
Life of this patent
Estimating the term…
Abstract
The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies.
Claims19
A method of producing a cell library expressing heterodimeric molecules on the cell surface, comprising:
- (a) fusing a first population of cells that express a first polypeptide with a second population of cells that comprise a plurality of polynucleotides that encode for a plurality of second polypeptides;
- wherein the first polypeptide comprises (i) an extracellular portion comprising an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains;
- and (ii) a non-immunoglobulin transmembrane portion, wherein the first polypeptide comprises a sequence at least 80% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:31, or SEQ ID NO:32;
- and wherein the second polypeptide comprises an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains;
- (b) incubating the fused cells to allow expression of heterodimeric molecules on the surface of the cells, wherein the heterodimeric molecules comprise the first polypeptide and one of the second polypeptides.
2 more elements
Dependencies are inferred from the claim text.