Method for screening for compound capable of enhancing or inhibiting OATP1B1 transport activity, and method for determining expression level of OATP1B1
AssigneeEisai R&D Management Co., Ltd.
InventorsSaki Izumi, Takafumi Komori, Yoshitane Nozaki
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Abstract
The present invention provides a method of screening for a compound that enhances or inhibits the transport activity of OATP1B1, using dichlorofluorescein. The present invention also provides a use of dichlorofluorescein in measurement of the expression level of OATP1B1. The present invention further provides a method for measuring the expression level of OATP1B1 in test cells, using dichlorofluorescein. The present invention further provides a use of a kit including dichlorofluorescein and positive cells expressing OATP1B1 in measurement of the expression level of OATP1B1 in test cells.
Claims13
A method of screening for a compound that enhances or inhibits a transport activity of OATP1B1, comprising:
- a step of preparing a cell expressing OATP1B1;
- a step of bringing dichlorofluorescein and a test compound into contact with the cell;
- a step of measuring a fluorescence intensity of dichlorofluorescein taken up by the cell;
- and
- a step of determining the test compound as a compound that enhances the transport activity of OATP1B1 in a case that the measured fluorescence intensity is higher than that when the test compound is not present and determining the test compound as a compound that inhibits the transport activity of OATP1B1 in a case that the measured fluorescence intensity is lower than that when the test compound is not present.
A method for measuring an expression level of OATP1B1 in a test cell, comprising:
- a step of bringing dichlorofluorescein into contact with the test cell;
- a step of measuring a fluorescence intensity of dichlorofluorescein taken up by the test cell;
- and
- a step of evaluating an expression level of OATP1B1 in the test cell based on the measured fluorescence intensity.
A kit comprising dichlorofluorescein and a positive cell expressing OATP1B1.
Dependencies are inferred from the claim text.