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US 8,945,859 B2Granted
Production of isobutanol by a microorganism with enhanced acetolactate synthase activity
AssigneeButamax Advanced Biofuels LLC
InventorsGail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Abstract
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
Claims17
A method comprising:
- (a) determining the acetolactate synthase (ALS) activity of a cell free extract prepared from a host cell expressing a heterologous ALS enzyme;
- and
- (b) expressing a heterologous DNA molecule encoding the ALS enzyme in a recombinant microorganism expressing an engineered isobutanol biosynthetic pathway,
- wherein the cell free extract comprises higher ALS activity than a control cell free extract prepared from a host cell not expressing the heterologous ALS enzyme, and
- wherein the recombinant microorganism is capable of bioproducing isobutanol suitable for use in fuel cells or vehicles.
2 more elements
Dependencies are inferred from the claim text.