Method for electrochemical detection of binding reactions
AssigneeFraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.
InventorsJulia Ettlinger, Nenad Gajovic-Eichelmann, Burkhard Micheel, Gudrun Scharte, Jörg Schenk
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Abstract
A method for performing homogeneous immunoassay formats with electrochemical detection in solution includes combining two different conjugates as reagents with a sample or a sample/buffer mixture, one conjugate including a redox marker and an analyte molecule and the second conjugate including an anti-redox marker antibody or a specifically binding fragment thereof and a molecule specifically binding the analyte.
Claims14
A method for performing homogeneous immunoassay formats with electrochemical detection in solution for the detection of an analyte in a sample comprising:
- (a) combining a water-soluble first conjugate and a second conjugate as reagents with the sample or the sample in a buffer solution, the first conjugate comprising a redox marker and the analyte conjugated via a linker and the second conjugate comprising an anti-redox marker antibody or a fragment that binds the redox marker and a molecule specifically binding the analyte conjugated via a linker,
- (b) binding the first conjugate and the second conjugate, whereby a detectable signal is generated or changed, and
- (c) detecting the signal;
- wherein an affinity constant of the molecule specifically binding the analyte is greater than an affinity constant of the anti-redox marker antibody or fragment that binds the redox marker.
Dependencies are inferred from the claim text.