Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides
AssigneeThe University of Chicago
InventorsOlaf Schneewind, Alice G. Cheng, Dominique M. Missiakas, Hwan Keun Kim
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Abstract
The present invention concerns methods and compositions for treating or preventing a bacterial infection, particularly infection by a Staphylococcus bacterium. The invention provides methods and compositions for stimulating an immune response against the bacteria. In certain embodiments, the methods and compositions involve an Ebh antigen.
Claims21
A method for eliciting an immune response against a staphylococcus bacterium in a subject comprising administering to the subject an effective amount of a staphylococcal ECM-binding protein homologue (Ebh) antigen and a staphylococcal Protein A (SpA) variant, wherein the Ebh antigen comprises amino acids 40-471 of SEQ ID NO:
- 24 or amino acids 2087-2544 of SEQ ID NO:
- 24 and the SpA variant comprises at least an amino acid substitution at one or more of positions 9, 10, 36, and 37 in Domain D.
A method of treating staphylococcal infection comprising the step of administering a vaccine comprising an isolated staphylococcal ECM-binding protein homolog (Ebh) antigen and a staphylococcal Protein A (SpA) variant, to a patient in need thereof, wherein the Ebh antigen comprises amino acids 40-471 of SEQ ID NO:
- 24 or amino acids 2087-2544 of SEQ ID NO:
- 24 and the SpA variant comprises at least an amino acid substitution at one or more of positions 9, 10, 36, and 37 in Domain D.
A method of reducing staphylococcal load in kidney tissue in a subject having or suspected of having a staphylococcal infection comprising administering an effective amount of a composition comprising a staphylococcal ECM-binding protein homologue (Ebh) antigen and a staphylococcal Protein A (SpA) variant, wherein the Ebh antigen comprises amino acids 40-471 of SEQ ID NO:
- 24 or amino acids 2087-2544 of SEQ ID NO:
- 24 and the SpA variant comprises at least an amino acid substitution at one or more of positions 9, 10, 36, and 37 in Domain D.
Dependencies are inferred from the claim text.