Process for producing poxviruses and poxvirus compositions
AssigneeTransgene S.A.
InventorsDaniel Malarme, Yves Cordier, Claude Sene
Life of this patent
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Abstract
The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.
Claims9
A medicament comprising
- (1) a pharmaceutically acceptable carrier, and
- (2) a therapeutically-effective amount of a composition of a recombinant poxvirus with no targeted infection specificity,
- wherein said poxvirus composition comprises intracellular mature virus (“IMV”) and extracellular enveloped virus (“EEV”) with more than 1% EEV, and
- wherein said poxvirus composition has been produced by a process, free from animal products and that does not use nuclease, comprising the steps of:
- a) infecting packaging cells to obtain infected cells,
- b) culturing said infected cells for an appropriate:
- period of time to produce poxviral particles,
- c) recovering said poxviral particles from the culture supernatant and from packaging cells disrupted by high speed homogenizer, to obtain a poxviral particle mixture,
- d) clarifying said poxviral particle mixture, thereby removing cellular debris, wherein said clarifying comprises depth filtration, to obtain a clarified poxviral particle mixture,
- e) concentrating said clarified poxviral particle mixture, wherein said concentrating comprises microfiltration, to obtain a concentrated poxviral particle mixture, and
- f) diafiltrating said concentrated poxviral mixture.
A composition of a recombinant poxvirus with no targeted infection specificity comprising intracellular mature virus (“IMV”) and extracellular enveloped virus (“EEV”) with more than 1% EEV, for the therapeutic or prophylactic treatment of cancer, wherein said poxvirus composition has been produced by a process, free from animal products and that does not use nuclease, comprising the steps of:
- a) infecting packaging cells to obtain infected cells,
- b) culturing said infected cells for an appropriate period of time to produce poxviral particles,
- c) recovering said poxviral particles from the culture supernatant and from packaging cells disrupted by high speed homogenizer, to obtain a poxviral particle mixture,
- d) clarifying said poxviral particle mixture, thereby removing cellular debris, wherein said clarifying comprises depth filtration, to obtain a clarified poxviral particle mixture,
- e) concentrating said clarified poxviral particle mixture, wherein said concentrating comprises microfiltration, to obtain a concentrated poxviral particle mixture, and
- f) diafiltrating said concentrated poxviral mixture.
Dependencies are inferred from the claim text.