Methods of treating inflammatory or thrombotic conditions with anti-P-selectin antibodies

A method of treating or inhibiting an inflammatory or thrombotic condition or disorder in a subject in need of such therapy, comprising:

• administering to the subject a therapeutically-effective amount of an antibody or binding fragment thereof which specifically binds to a conformational epitope of P-selectin which is within amino acid positions 1-35 of SEQ ID NO:1,
• wherein the antibody or binding fragment thereof has the ability to block the binding of P-selectin glycoprotein ligand-1 (PSGL-1) to P-selectin and the ability to cause dissociation of a preformed P-selectin-PSGL-1 complex;
• wherein the amino acids in amino acid positions 4, 14, 17, 21, and 22 are H, I, K, N, and R, respectively;
• provided that the antibody or binding fragment thereof is not G1 antibody, the G1 antibody comprising variable light chains each individually having an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 47, 51, 53, 55, and 67, and further comprising variable heavy chains each individually having an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 61, 65, and 69;
• further provided that the antibody or binding fragment thereof is not hSel001 antibody comprising variable light chains each individually having an amino acid sequence of SEQ ID NO:40 and variable heavy chains each individually having an amino acid sequence of SEQ ID NO:44;
• further provided that the antibody or binding fragment thereof is not a binding fragment of the G1 antibody;
• or a binding fragment of the hSel001 antibody.