Controlled activation of the reuterin-production machinery of Lactobacillus
AssigneeBiogaia AB
InventorStefan Roos
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Abstract
Methods for the controlled activation of the reuterin-production machinery of Lactobacillus reuteri by adding glycerol and other substances during the manufacture of cell-cultures and keeping the produced reuterin in the bacterial cell during preservation and storage. In particular this invention relates to the manufacture of large amounts of L. reuteri that are loaded with reuterin, and the use of such loaded bacteria for applications such as prevention and treatment of diseases, for food applications and the like.
Claims5
A product comprising freeze-dried Lactobacillus reuteri cells containing microcompartments loaded with reuterin, the product is produced by a method for the controlled activation of the reuterin-production machinery of Lactobacillus reuteri cells, the method comprising:
- culturing Lactobacillus reuteri cells;
- adding glycerol to the Lactobacillus reuteri cells during the cultures, so that the Lactobacillus reuteri cells form microcompartments and produce reuterin therein, and further adding glycerol to the Lactobacillus reuteri cultures after said reuterin production and prior to freeze-drying, wherein addition of glycerol to the Lactobacillus reuteri cells in the culture that has produced reuterin enables Lactobacillus reuteri cells to survive in the product after freeze-drying;
- wherein the microcompartments enable the reuterin to be retained inside the Lactobacillus reuteri cells during the preservation and storage of the Lactobacillus reuteri in an amount greater than in cells lacking microcompartments.
A product comprising freeze-dried Lactobacillus reuteri cells containing microcompartments loaded with reuterin, the product is produced by a method for the controlled activation of the reuterin-production machinery of Lactobacillus reuteri cells, the method comprising:
- manufacturing Lactobacillus reuteri cell cultures;
- wherein the manufacture of the cell cultures comprises:
- fermenting the cell cultures;
- washing the cell cultures;
- and
- freeze-drying the cell cultures;
- and
- wherein glycerol is added to the Lactobacillus reuteri cultures after production of reuterin by Lactobacillus reuteri and before freeze-drying, wherein about 1 to about 500 mM of glycerol is added to the Lactobacillus reuteri cell cultures prior to freeze-drying the cell cultures, so that the Lactobacillus reuteri cells form microcompartments and produce reuterin therein, wherein the microcompartments enable the reuterin to be retained inside the Lactobacillus reuteri cells during preservation and storage of the Lactobacillus reuteri in an amount greater than in cells lacking microcompartments, and wherein addition of glycerol to the Lactobacillus reuteri cell culture that has produced reuterin enables Lactobacillus reuteri cells to survive in the product after freeze-drying.
Dependencies are inferred from the claim text.