DNA sequence, and recombinant preparation of the grass pollen allergen Lol p4
AssigneeMerck Patent GmbH
InventorsAndreas Nandy, Helmut Fiebig, Oliver Cromwell
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Abstract
The present invention relates to the provision of a DNA sequence of the major grass pollen allergen Lol p 4. The invention also encompasses fragments, new combinations of partial sequences and point mutants having a hypoallergenic action. The recombinant DNA molecules and the derived polypeptides, fragments, new combinations of partial sequences and variants can be utilised for the therapy of pollen-allergic diseases. The proteins prepared by recombinant methods can be employed for in vitro and in vivo diagnosis of pollen allergies.
Claims6
An expression vector or a cloning system comprising a polynucleotide comprising the nucleic acid sequence set forth in SEQ ID NO:
- 1 which is functionally linked to a heterologous expression control sequence.
An isolated DNA comprising the nucleic acid sequence which encodes the polypeptide sequence set forth in SEQ ID NO:
- 2, wherein said nucleic acid sequence has been codon optimized for the expression of said polypeptide of SEQ ID NO:
- 2 by recombinant methods in a host cell that is not a cell of Lolium perenne.
Dependencies are inferred from the claim text.