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US 8,945,511 B2Granted
Sensitive methods for detecting the presence of cancer associated with the over-expression of galectin-3 using biomarkers derived from galectin-3
OwnerPaul Weinberger, John M. Koomen +3inventors
InventorsPaul Weinberger, John M. Koomen, Willam Shelley Dynan, David James Terris, Mark Asher Markley
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Abstract
Described herein are sensitive methods for determining if a subject has cancer. The methods generally involve quantifying the amount of one or more biomarkers derived from Galectin-3 in a biological sample from the subject by mass spectrometry, wherein an increase in the amount of one or more biomarkers in the biological sample as compared to a control is an indication of the presence of cancer in the subject.
Claims5
A method for determining if a subject has thyroid cancer, the method comprising
- (a) contacting a thyroid aspirate derived from the subject comprising galectin-3 with trypsin to digest galectin-3 and produce one or more biomarkers selected from the group consisting of SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, and any combination thereof,
- (b) adding a control biomarker to the thyroid aspirate, wherein the control biomarker is SEQ ID NO 5 or SEQ ID NO 6,
- (c) quantifying the amount of the biomarker in the thyroid aspirate by multiple reaction monitoring, and
- (d) comparing the amount of the biomarker in the thyroid aspirate to the amount of the same biomarker from a second thyroid aspirate from a subject that does not have thyroid cancer,
- wherein an increase in the amount of the biomarker in the thyroid aspirate as compared to the amount of the same biomarker from the second thyroid aspirate from the subject that does not have thyroid cancer is an indication of the presence of thyroid cancer in the subject.
2 more elements
Dependencies are inferred from the claim text.