Apparatus and methodology to carry out biochemical testing on a centrifugal platform using flow splitting technique

An apparatus using flow splitting technique to carry out antibody-related tests comprises a rotator platform and a microfluidic disc set on the rotator platform, both shaped like a disc, round and flat;

• the microfluidic disc comprising at least two layers of microfluidic structures, wherein each layer of the at least two layers of microfluidic structures, from the center to the periphery, comprising:
• an injection chamber with a reagent injection orifice, wherein the injection chamber is configured on the center of the microfluidic disc and the center of the at least two layers of the microfluidic structures, and wherein the reagent injection orifice is also configured on the center of the microfluidic disc;
• at least one microfluidic channel arranged radially in the at least two layers of the microfluidic structures and connected with the injection chamber, the at least one microfluidic channel used as the channel for liquid to flow through and for reagents flowing from the center of the at least two layers of microfluidic structures to the periphery;
• at least one detection chamber with a sample injection orifice placed at the periphery of the at least two layers of the microfluidic structures for the reagents to carry out reactions and connected with each at least one microfluidic channel placed in the different layers but at the same position corresponding to that in an upper layer and a lower layer of the at least two layers of the microfluidic structures;
• and
• at least one waste chamber set next to and connected to the at least one detection chamber for waste storage after the reactions;
• wherein
• the at least one microfluidic channel comprising:
• a splitting chamber set outside and connected with the injection chamber, equally splitting the reagents from the injection chamber;
• and
• a herringbone shape microvalve set between and connected with the splitting chamber and the at least one detection chamber, controlling the reagents flowing from the splitting chamber to the at least one detection chamber.