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US 8,945,360 B2Granted
High-performing electrophoresis gels with extended shelf lives
AssigneeBio-Rad Laboratories, Inc.
InventorsShane Petersen, Cory Panattoni, Craig Rowell
Life of this patent
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Abstract
Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.
Claims32
A polyacrylamide gel comprising:
- a buffer selected from the group consisting of tris(hydroxymethyl)aminomethane and bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and
- taurine at a concentration of from about 100 mM to about 300 mM,
- in a gel matrix consisting of crosslinked polyacrylamide, said polyacrylamide gel having a pH of from about 6.4 to about 7.0.
A pre-mixed solution for casting to form a polyacrylamide electrophoresis gel, said pre-mixed solution comprising the following ingredients dissolved in water:
- (i) acrylamide monomer,
- (ii) an acrylamide crosslinker,
- (iii) a buffer selected from the group consisting of tris(hydroxymethyl)aminomethane and bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and
- (iv) taurine,
- wherein said taurine is at a concentration of from about 100 mM to about 300 mM in said pre-mixed solution, and said pre-mixed solution has a pH of from about 6.4 to about 7.0.
2 more elements
Dependencies are inferred from the claim text.