Method of enhancing 3D image information density
AssigneeNational Tsing Hua University
InventorsAnn-Shyn Chiang, Hsiu-Ming Chang, Yung-Chang Chen, Kuan-Yu Chen
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Abstract
A method of enhancing 3D image information density, comprising providing a confocal fluorescent microscope and a rotational stage. 3D image samples at different angles are collected. A deconvolution process of the 3D image samples by a processing unit is performed. A registration process of the deconvoluted 3D image samples by the processing unit is performed. An interpolation process of the registered 3D image samples by the processing unit is performed to output a 3D image in high resolution.
Claims8
A method of enhancing final 3D image information density by fusing images stacks acquired at different observation angles, comprising:
- performing a deconvolution process for each of a plurality of 3D image samples acquired from different observation angles, defined by rotation angles of a revolvable stage, by a processing unit designed to recover the distortion caused by a point spread function, wherein said performing a deconvolution process comprises performing a blind deconvolution to find said point spread function and the reconstructed undistorted 3D optical slices from said 3D image samples;
- performing a registration process of said deconvoluted plurality of 3D image samples acquired from different observation angles by said processing unit, wherein said performing a registration process comprises generating SIFT (Scale Invariant Feature Transform) matched features of every image of said plurality of 3D image samples;
- and
- performing an interpolation process of said registered 3D image samples acquired from different observation angles by said processing unit to output a final 3D image with increased depth resolution in said final 3D image;
- wherein said performing an interpolation process comprises performing a searching process, and performing an intensity rectification process to fix artifacts generated from an intensity-based interpolation;
- wherein said performing an intensity rectification process comprises providing a weighting function to maintain continuous intensity changes between upper slice and lower slice and a rectification function to satisfy said continuous intensity changes.
Dependencies are inferred from the claim text.