Plural third harmonic generation microscopic system and method

A plural third harmonic generation (THG) microscopic system for retrieving spatial distribution microscopic images and characteristics of bio-molecules, comprising:

• a laser device, for irradiating a laser beam with a plurality of different wavelengths;
• a scanning device for receiving the laser beam with the plurality of different wavelengths, producing a two-dimensional scanning laser beam and directing the laser beam onto a reflective minor;
• a beam splitter device for receiving the laser beam reflected from the reflective minor and directing the reflected laser beam to a microscopic device;
• the microscopic device, including an object lens, for focusing the laser beam with the plurality of different wavelengths and irradiating the same onto an observational sample of the bio-molecules, thereby producing a THG signal for each wavelength responded from the observational sample, in which a THG beam with the THG response signal for each wavelength is incident on the object lens, irradiated to the beam splitter device, and split and irradiated onto a lens;
• a spectrometer, for processing the THG response signal for each wavelength, which response signal for each wavelength is each converted into an analog signal;
• an Analog/Digital converter, for converting the analog signal for each wavelength into a digital image signal and/or spectrum signal;
• and
• a computer system for receiving the digital image signal and/or spectrum signal and producing a plurality of THG image signals with different wavelengths or a THG spectrum.