Nonlinear luminescent molecule, fluorescent stain, and observation method
AssigneeOsaka University
InventorsKatsumasa Fujita, Shin Mizukami, Kazuya Kikuchi, Satoshi Kawata, Shogo Kawano
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Abstract
The present invention relates to a nonlinear fluorescent molecule that generates a nonlinear fluorescence reaction by incidence of excitation light. This nonlinear fluorescence molecule includes donors and, and an acceptor that is coupled to the donors and. As the donor is excited by the incidence of the excitation light, electric charge moves from the donor to the acceptor. Then, the donor and the acceptor form a charge separated state. In a state in which the charge separated state is maintained, the donor fluoresces when transiting from an excited state to a ground state.
Claims10
A molecule that generates nonlinear fluorescence, of formula:
A florescent stain, comprising a molecule of formula
- dissolved in a solvent and generates nonlinear fluorescence in the presence of a sample that is being observed for nonlinear fluorescence under excitation light.
A method of imaging a sample using nonlinear fluorescence, comprising:
- dying a sample with a stain containing a molecule of formula
- dissolved in a solvent,
- irradiating the dyed sample with a laser beam to generate nonlinear fluorescence,
- scanning, to change position of the laser beam relative to the sample,
- separating fluorescence generated by said molecule and detecting it, and
- imaging the sample based on intensity of the detected fluorescence.
Dependencies are inferred from the claim text.