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US 8,940,869 B2Granted
Method and system for detecting and differentiating cancer and sepsis in mammals using biomarkers
AssigneeVeterinary Diagnostics Institute, Inc.
InventorRandy Ringold
Life of this patent
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Abstract
The invention provides a method and system for developing and using diagnoses of cancer and sepsis in canine subjects using Thymidine kinase (TK), c-reactive protein (CRP), and C-type natriuretic peptide (CNP) as biomarkers. The level of each biomarker may be measured and an index may be computed using a two- or a three-biomarker method. The invention provides a predefined scale for the index where each range of the index matches a health condition. The latter allows a practitioner, through computing an index value of a patient, to determine the health status of the patient by comparing the index value to the predefined scale.
Claims2
A method for detecting sepsis in canine subjects comprising the steps of:
- obtaining a blood serum sample from a canine subject;
- obtaining the concentration of c-reactive protein in said blood serum sample and assigning a first discrete value to said c-reactive protein concentration, wherein said first discrete value is assigned a value:
- zero (0) when the concentration of c-reactive protein is less than 40 mg/l,
- one (1) when the concentration of c-reactive protein is between 40.1 mg/l and 56.4 mg/l, or
- two (2) when the concentration of c-reactive protein is greater than 56.5 mg/l;
- obtaining the concentration of C-type natriuretic peptide in said blood serum sample and assigning a second discrete value to said C-type natriuretic peptide concentration, wherein said second discrete value is assigned the value:
- zero (0) when said concentration of C-type natriuretic peptide is less than 3.8 picomole/l,
- one (1) when the concentration of C-type natriuretic peptide is between 3.9 picomole/l and 13.3 picomole/l,
- two (2) when the concentration of C-type natriuretic peptide is between 13.4 picomole/l and 20 picomole/l, or
- three (3) when the concentration of C-type natriuretic peptide is greater than 20.1 picomole/l;
- computing an index value by adding:
- the first discrete value multiplied by a c-reactive protein weighing coefficient of value 1.43, and
- the second discrete value multiplied by a C-type natriuretic peptide weighing coefficient of value 1.17, and
- determining that the canine subject is a carrier of sepsis if said index value is above a criterion value of 2.86.
11 more elements
A method for detecting sepsis in canine subjects comprising the steps of:
- obtaining a blood serum sample from a canine subject;
- obtaining the enzymatic level of thymidine kinase in the blood serum sample sample and assigning a first discrete value to said thymidine kinase activity, wherein said first discrete value is assigned a value:
- zero (0) when the level of thymidine kinase activity is less than 4.2 U/I,
- one (1) when the level of thymidine kinase activity is between 4.2 U/I and 13.3 U/I, or
- two (2) when the level of thymidine kinase activity is greater than 13.4 U/I;
- obtaining the concentration of c-reactive protein in said blood serum sample and assigning a second discrete value to said c-reactive protein concentration, wherein said second discrete value is assigned a value:
- zero (0) when the concentration of c-reactive protein is less than 40 mg/l,
- one (1) when the concentration of c-reactive protein is between 40.1 mg/l and 56.4 mg/l, or
- two (2) when the concentration of c-reactive protein is greater than 56.5 mg/l;
- obtaining the concentration of C-type natriuretic peptide in said blood serum sample and assigning a third discrete value to said C-type natriuretic peptide concentration, wherein said third discrete value is assigned the value:
- zero (0) when said concentration of C-type natriuretic peptide is less than 3.8 picomole/l,
- one (1) when the concentration of C-type natriuretic peptide is between 3.9 picomole/l and 13.3 picomole/l,
- two (2) when the concentration of C-type natriuretic peptide is between 13.4 picomole/l and 20 picomole/l, or
- three (3) when the concentration of C-type natriuretic peptide is greater than 20.1 picomole/l;
- computing an index value by adding:
- the first discrete value multiplied by a thymidine kinase weighing coefficient of value 0.27,
- the second discrete value multiplied by a c-reactive protein weighing coefficient of value 1.47, and
- the third discrete value multiplied by a C- type natriuretic peptide weighing coefficient of value 1, and
- determining that the canine subject is a carrier of sepsis if said index value is above a criterion value of 2.94.
16 more elements
Dependencies are inferred from the claim text.