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US 8,940,663 B2Granted
Nano-scale biosensors
AssigneeBoard of Regents, The University of Texas System
InventorsSamir M. Iqbal, Swati Goyal, Young-tae Kim, Yuan Wan
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Abstract
Devices, systems, and methods for detecting protein-nucleic acid and cell-nucleic acid hybridization, using surface-tethered aptamer probes, without the use of labeling or target modification and capable of recycling.
Claims27
A device, comprising:
- a thermally responsive, electrically insulating substrate;
- at least one heating element;
- and
- a first detecting unit, comprising:
- a first electrode and a second electrode separated by a nanogap;
- and
- a plurality of first aptamer probes attached to said substrate in said nanogap.
4 more elements
A method for detecting hybridization of a target, comprising:
- providing a device, comprising:
- a thermally responsive, electrically insulating substrate;
- at least one heating element;
- and
- a first detecting unit, comprising:
- a first electrode and a second electrode separated by a nanogap;
- and
- a plurality of first aptamer probes attached to said substrate in said nanogap;
- providing a solution comprising said target under hybridizing conditions;
- wherein said target is a protein or a cell;
- and
- wherein said target hybridizes at least some of said first aptamer probes;
- applying a voltage drop across said electrodes;
- and
- measuring a change in conductivity, resistivity, capacitance, or impedance across said electrodes at known locations to determine specific binding of said target to said first aptamer probes.
12 more elements
Dependencies are inferred from the claim text.