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US 8,940,534 B2Granted
Immortalized avian cell lines for virus production
AssigneeProbiogen AG
InventorsVolker Sandig, Ingo Jordan
Life of this patent
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Abstract
The present invention relates to immortalized avian cell lines suitable for production of biologicals or viruses for vaccination. In particular, the cell lines are derived from primary cells which are transformed with at least two viral or cellular genes, one of which causes cell cycle progression whereas the other interferes with innate protective mechanisms of the cell induced by dysregulated replication. The invention moreover relates to the production of said immortalized cell lines and their use for producing biologicals or viruses for vaccination.
Claims17
An immortal duck cell line comprising:
- (i) a first viral gene which is a Mastadenovirus early region 1A (E1A) gene wherein said E1A gene has the sequence of bp 1193 to 2309 of SEQ ID NO:
- 7 or the sequence complementary to bp 4230 to 3113 of SEQ ID NO:
- 9, wherein the expression of said first gene is driven by a human phosphoglycerate kinase (PGK) promoter;
- and
- (ii) a second viral gene which codes for a Mastadenovirus early region 1B 55K (E1B 55K) protein wherein said E1B gene has the sequence of bp 1145 to 3007 of SEQ ID NO:
- 8 or the sequence complementary to bp 2345 to 550 of SEQ ID NO:
- 9, wherein the expression of said second gene is driven by a mouse cytomegalovirus (CMV) immediate early promoter or a human herpesvirus thymidine kinase (tk) promoter, and wherein said first and second viral genes are integrated and stably expressed in said duck cell line.
4 more elements
Dependencies are inferred from the claim text.