Mouse artificial chromosome vector
AssigneesNational University Corporation Tottori University, Chromocenter Inc.
InventorsMitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
Life of this patent
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Abstract
Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.
Claims32
A mouse artificial chromosome vector, comprising:
- a natural centromere derived from a mouse chromosome 11;
- a mouse-chromosome-derived long-arm fragment formed by deleting at least about 99% of all endogenous genes from the long-arm distal region proximal to the centromere of mouse chromosome 11;
- a telomere sequence so as to form the mouse artificial chromosome vector contained in the deposited cell line DT40 B6bT-1 (FERM BP-11128) wherein the vector is stably retained in cells and tissues of a mammal at a retention rate of about 90% or more.
Dependencies are inferred from the claim text.