Method of producing a lipolytic enzyme

A Trichoderma reesei cell comprising:

• a promoter region of a Trichoderma reesei cellobiohydrolase 1;
• and
• a sequence comprising one or more of:
• (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme protein having at least 95% sequence identity to SEQ ID NO:
• 1 or 2;
• (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or a nucleotide sequence which is at least 95% sequence identity to SEQ ID NO:
• 3 or SEQ ID NO:
• 4;
• or
• (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises an the nucleotide sequence which hybridizes to SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or a nucleotide sequence which is at least 95% sequence identity to SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or the complement of any thereof under high stringency conditions of 0.1 SSC (0.15 M NaCl, pH 7.0) at 65° C.

A method of producing an active lipolytic enzyme by a Trichoderma reesei cell to use in foodstuff, the method comprising:

• transforming or transfecting the Trichoderma reesei cell having a promoter region of a Trichoderma reesei cellobiohydrolase 1, the Trichoderma reesei cell comprising one or more of:
• (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO:
• 1 or SEQ ID NO:
• 2 or an amino acid sequence which has at least 95% sequence identity to SEQ ID NO:
• 1 or 2;
• (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or a nucleotide sequence which is at least 95% sequence identity to SEQ ID NO:
• 3 or SEQ ID NO:
• 4;
• or
• (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or a nucleotide sequence which is at least 95% sequence identity to SEQ ID NO:
• 3 or SEQ ID NO:
• 4 or the complement of any thereof under high stringency conditions of 0.1 SSC (0.15 M NaCl, pH 7.0) at 65° C.;
• for improving one or more of the following:
• expression of the lipolytic enzyme, glycosylation of the lipolytic enzyme, enzyme activity, or yield.