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US 8,940,514 B2Granted
Thioesterase and a method of producing fatty acids or lipids using the thioesterase
AssigneeKao Corporation
InventorsTakuto Tojo, Keiji Endo
Life of this patent
Estimating the term…
Abstract
A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.
Claims15
A recombinant vector comprising an isolated cDNA, the isolated cDNA comprising any one of the following DNAs (a) to (f):
- (a) DNA encoding a protein that comprises an amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (b) DNA encoding a protein that comprises an amino acid sequence in which one to two amino acids are deleted, substituted, inserted and/or added in the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (c) DNA encoding a protein that comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity;
- (d) DNA comprising the nucleotide sequence set forth in SEQ ID NO:
- 2, which codes for a protein having thioesterase activity,
- (e) DNA capable of hybridizing with a DNA comprising a complementary sequence to the nucleotide sequence set forth in SEQ ID NO:
- 2 when incubated in a solution containing 6 x SSC, 0.5% SDS, 5x Denhardt and 100 mg/mL herring sperm DNA at 65° C. for 8 to 16 hours, and which codes for a protein having thioesterase activity, and
- (f) DNA comprising a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2, which codes for a protein having thioesterase activity.
9 more elements
A transformant that has been transformed with DNA or with a recombinant vector, said DNA or said recombinant vector comprising
- (a) A cDNA encoding a protein that comprises the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (b) A cDNA encoding a protein that comprises an amino acid sequence in which one to two amino acids are deleted, substituted, inserted and/or added in the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (c) A cDNA encoding a protein that comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (d) A DNA comprising the nucleotide sequence set forth in SEQ ID NO:
- 2, which codes a protein having thioesterase activity,
- (e) A DNA capable of hybridizing with a DNA comprising a complementary sequence to the nucleotide sequence set forth in SEQ ID NO:
- 2 when incubated in a solution containing 6 x SSC, 0.5% SDS, 5x Denhardt and 100 mg/mL herring sperm DNA at 65° C. for 8 to 16 hours, and which codes a protein having thioesterase activity, and
- (f) A DNA comprising a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2, which codes a protein having thioesterase activity.
9 more elements
A method of enhancing productivity of a fatty acid or a lipid containing a fatty acid, comprising introducing a cDNA that comprises any one of the following (a) to (f) into a host:
- (a) A cDNA that encodes a protein that comprises the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (b) A cDNA that encodes a protein that comprises an amino acid sequence in which one to two amino acids are deleted, substituted, inserted and/or added in the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (c) A cDNA that encodes a protein that comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said protein has thioesterase activity,
- (d) A DNA comprising a nucleotide sequence set forth in SEQ ID NO:
- 2, which codes a protein having thioesterase activity,
- (e) A DNA capable of hybridizing with a DNA comprising a complementary sequence to the nucleotide sequence set forth in SEQ ID NO:
- 2 when incubated in a solution containing 6 x SSC, 0.5% SDS, 5x Denhardt and 100 mg/mL herring sperm DNA at 65° C. for 8 to 16 hours, and which codes a protein having thioesterase activity, and
- (f) A DNA comprising a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 2, which codes a protein having thioesterase activity.
9 more elements
Dependencies are inferred from the claim text.