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US 8,940,512 B2Granted
Processes for synthesizing alkaline phosphatase conjugates
AssigneeShenzhen Mindray Bio-Medical Electronics Co., Ltd.
InventorsChungen Qian, Ke Li, Yuping Zhang
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Abstract
Methods for synthesizing an ALP conjugate are provided. The methods may include activating a carboxyl group of the ALP with a carbodiimide, to generate an active ester; and adding the substance to be conjugated such that a synthetic reaction occurs between the active ester and the substance to be conjugated, to generate an ALP conjugate.
Claims12
A method of synthesizing an alkaline phosphatase (“ALP”) conjugate, comprising:
- activating a carbonyl group of ALP with a carbodiimide under acidic conditions to form an active ALP ester;
- and
- adding a substance to be conjugated under basic conditions;
- wherein the active ALP ester reacts with the substance to be conjugated to generate an ALP conjugate.
1 more element
A method of synthesizing an enzyme conjugate, comprising:
- activating a carbonyl group of an enzyme with a carbodiimide, under acidic conditions between about pH 4.0 and about pH 5.0, to form an active ester;
- and
- adding a substance to be conjugated selected from at least one of the following:
- a peptide, a protein, an antigen, an antibody, and Staphylococcus protein A, under basic conditions between about pH 7.5 and about pH 9.0;
- wherein the active ester reacts with the substance to be conjugated to generate an enzyme conjugate.
2 more elements
Dependencies are inferred from the claim text.