Microorganisms and methods for the production of caprolactone
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Robin E. Osterhout, Priti Pharkya, Mark J. Burk
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Abstract
The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.
Claims20
A non-naturally occurring microbial organism, said microbial organism having a caprolactone pathway and comprising at least one exogenous nucleic acid encoding a caprolactone pathway enzyme expressed in a sufficient amount to produce caprolactone, wherein said caprolactone pathway comprises a pathway selected from:
- (1) 1D, 1A, 1B, and 1C;
- (2) 1E, 1B, 1C and 1D;
- (3) 1F, 1A, 1B, 1C and 1D;
- (4) 1F, 1E, 1B, 1C and 1D;
- (5) 1A, 1B and 1G;
- (6) 1E, 1B and 1G;
- (7) 1F, 1A, 1B and 1G;
- (8) 1F, 1E, 1B and 1G;
- (9) 1A, 1B, 1C, 1J and 1I;
- (10) 1E, 1B, 1C, 1J and 1I;
- (11) 1F, 1A, 1B, 1C, 1J and 1I;
- (12) 1F, 1E, 1B, 1C, 1J and 1I;
- (13) 1A, 1B, 1H and 1I;
- (14) 1E, 1B, 1H and 1I;
- (15) 1F, 1A, 1B, 1H and 1I;
- (16) 1F, 1E, 1B, 1H and 1I;
- (17) 2A, 2B, 2C, 2D, 2E and 2F;
- (18) 2A, 2B, 2C, 2D and 2G;
- (19) 2A, 2B, 2C, 2D, 2E, 2J and 2I;
- (20) 2A, 2B, 2C, 2D, 2H and 2I;
- (21) 4A, 4B, 4C, 4D and 3A;
- and
- (22) 5A, 5B, 5C an 3A,
- wherein 1A is an adipyl-CoA reductase, wherein 1B is an adipate semialdehyde reductase, wherein 1C is a 6-hydroxyhexanoyl-CoA transferase or a 6-hydroxyhexanoyl-CoA synthetase, wherein 1D is a 6-hydroxyhexanoyl-CoA cyclase or spontaneous cyclization, wherein 1E is an adipate reductase, wherein 1F is an adipyl-CoA transferase, an adipyl-CoA synthetase or an adipyl-CoA hydrolase, wherein 1G is a 6-hydroxyhexanoate cyclase, wherein 1H is a 6-hydroxyhexanoate kinase, wherein 1I is a 6-hydroxyhexanoyl phosphate cyclase or spontaneous cyclization, wherein 1J is a phosphotrans-6-hydroxyhexanoylase, wherein 2A is a 4-hydroxybutyryl-CoA:acetyl-CoA acyltransferase, wherein 2B is a 3-oxo-6-hydroxyhexanoyl-CoA reductase, wherein 2C is a 3,6-dihydroxyhexanoyl-CoA dehydratase, wherein 2D is a 6-hydroxyhex-2-enoyl-CoA reductase, wherein 2E is a 6-hydroxyhexanoyl-CoA transferase, a 6-hydroxyhexanoyl-CoA synthetase or a 6-hydroxyhexanoyl-CoA hydrolase, wherein 2F is a 6-hydroxyhexanoate cyclase, wherein 2G is a 6-hydroxyhexanoyl-CoA cyclase or spontaneous cyclization, wherein 2H is a phosphotrans-6-hydroxyhexanoylase, wherein 2I is a 6-hydroxyhexanoyl phosphate cyclase or spontaneous cyclization, wherein 2J is a 6-hydroxyhexanoate kinase, wherein 3A is a cyclohexanone monooxygenase, wherein 4A is an adipate semialdehyde dehydratase, wherein 4B is a cyclohexane-1,2-dione reductase, wherein 4C is a 2-hydroxycyclohexanone reductase, wherein 4D is a cyclohexane-1,2-diol dehydratase, wherein 5A is a 2-ketocyclohexane-1-carboxoyl-CoA hydrolase (acting on C—C), wherein 5B is a 2-ketocyclohexane-1-carboxoyl-CoA transferase, a 2-ketocyclohexane-1-carboxoyl-CoA synthetase or a 2-ketocyclohexane-1-carboxoyl-CoA hydrolase, and wherein 5C is a 2-ketocyclohexane-1-carboxylate decarboxylase.
Culture medium comprising bioderived caprolactone, wherein said bioderived caprolactone has a carbon-12, carbon-13 and carbon-14 isotope ratio that reflects an atmospheric carbon dioxide uptake source.
Bioderived caprolactone having a carbon-12, carbon-13 and carbon-14 isotope ratio that reflects an atmospheric carbon dioxide uptake source.
Dependencies are inferred from the claim text.