Real-time sequencing methods and systems
AssigneePacific Biosciences of California, Inc.
InventorsJonas Korlach, Stephen Turner, Eric Schadt
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Abstract
The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance or inhibit such reactions. In certain preferred embodiments, RNA templates are used in single-molecule real-time sequencing reactions.
Claims15
A method for identifying a nucleic acid modification in a single molecule RNA template, the method comprising:
- (a) providing a complex comprising a circularized form of the RNA template and a sequencing engine, wherein the complex is immobilized in an optical confinement;
- (b) introducing a set of labeled nucleotides to the optical confinement;
- (c) processing the circularized RNA template with the sequencing engine;
- (d) detecting an energetic profile of the sequencing engine during the processing step (c), thereby identifying a nucleic acid modification in the RNA template.
Dependencies are inferred from the claim text.