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US 8,940,506 B2Granted
High-sensitive fluorescent energy transfer assay using fluoresent amino acids and fluorescent proteins
AssigneeThe Regents of the University of California
InventorsJiayu Liao, Yang Song, Yongfeng Zhao, Yan Liu
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Abstract
The disclosure provides method and composition utilizing fluorescent amino acids and endogenous fluorescent proteins comprising a moiety capable of undergoing FRET. The methods and compositions of the disclosure are useful in analyzing protein structure and function, and screening molecular inhibitors.
Claims11
A polypeptide comprising:
- at least a first fluorescent amino acid comprising NBD (7-nitrobenzo-2-oxa-1,3-diazol-4-yl);
- and
- at least a second fluorescent amino acid comprising a coumarin fluorescent amino acid,
- wherein the at least first and second fluorescent amino acids are separated by 1, 2, 3, 4, 5, 6, 7, 8 or more amino acids and are capable of undergoing Föster resonance energy transfer (FRET).
1 more element
Dependencies are inferred from the claim text.