Method and system for the analysis of high density cells samples
AssigneesAccupath Diagnostic Laboratories, Inc., Biodot, Inc.
InventorsMathew Moore, Miriam Reyes, David Baunoch, Thomas C. Tisone, Brendan O'Farrell
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Abstract
Methods for forming cell arrays of multiple cell samples arranged substantially in a monolayer on a single substrate particularly suited for diagnostic analysis are disclosed. The cell arrays are formed with a high-speed dispensing apparatus capable of dispensing small volumes in precise, complex patterns. Also disclosed are substrates upon which cell arrays may be formed, and methods for conducting diagnostic analyzes on the formed cell arrays.
Claims29
A method of forming a cell array comprising a plurality of discrete cell samples on a single substrate, comprising:
- aspirating a cell suspension from a source;
- providing relative motion between the cell suspension and the substrate;
- metering a predetermined amount of the cell suspension using a direct current fluid source;
- dispensing the metered amount of the cell suspension in the form of droplets onto the substrate so as to form a plurality of discrete cell samples, each discrete cell sample being arranged substantially in a monolayer on the substrate, each discrete cell sample being spaced apart from each other on the substrate such that substantially no overlap or abutment occurs between adjacent cells within each of said discrete cell samples;
- and
- dispensing a predetermined amount of probe or stain to interact with at least one of the discrete cell samples.
Dependencies are inferred from the claim text.